Irreversible Heat Inactivation of Transfer Ribonucleic Acids
نویسندگان
چکیده
منابع مشابه
Isolation of "renaturable" transfer ribonucleic acids.
A new type of fractionating step involving gel Cltration is introduced for the purification of certain transfer ribonucleic acids (tRNAs). It is based on the increase that occurs in the molecular dimensions of these tRNAs when they are trapped, reversibly, in a denatured state under conditions where the other tRNA components of the mixture are in the native conformation. This step, coupled to c...
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1. HAGELBERG, L., Ber. de&. hem. Ges., 23,1083 (1890). 2. KATJFMANN. H. P.. GINDSBERG. E.. ROTTIG, W.. AND SALCHOW, R., Ber. $eut. chkm. Ges., 70, 2519 (1937). ’ ’ 3. SIEGEL, J. R., AND ROSENBLATT, D. H., J. Am. Chem. Sot., 80, 1753 (1958). 4. GABRIEL, S., Ber. deut. them. Ges., 22, 1139 (1889). 5. FIESER, i. I?., Experiments in organic chemistry, Ed. 2, D. C. Heath and Co., New York, 1941, p. ...
متن کاملIrreversible heat inactivation of DNase I without RNA degradation.
In many applications of RT-PCR, residual genomic DNA in total RNA preparations is amplified together with RNA, which results in false-positive data. One way to overcome this problem is to design primers that span a region containing one or more introns, thereby creating a diagnostic size difference between the amplification products originating from the RNA or the DNA. However, this method will...
متن کاملChromatographic separation of mononucleotides derived from transfer ribonucleic acids.
1. A method is described for fractionating alkaline and enzymic hydrolysates of ribonucleic acids and oligonucleotides on DEAE-cellulose columns by using a constant-composition eluent. 2. The relative partition coefficients of the four major and several minor nucleotides present in nucleic acid digests are given. 3. The influence of pH and of molarity of the buffer on the separation is describe...
متن کاملMethionine transfer ribonucleic acids of avian myeloblastosis virus.
Chick-embryo cells contain four isoaccepting species of methionine transfer RNA (I-IV). One species (I) is the initiator, tRNA(f) (Met), and the others (II, III, and IV) are the donors of internal methionyl residues (tRNA(m) (Met)). Over 85% of the tRNA(Met) in purified avian myeloblastosis virus consists of one tRNA(m) (Met) species, which resembles host-cell tRNA(Met) IV with respect to chrom...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96095-3